Analysis Note

ControlControl Histones (from Hela cells) colcemid-treated or untreated

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Anti-CENP-A Antibody is a Rabbit Polyclonal Antibody for detection of CENP-A also known as Centromere autoantigen A & has been tested in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CENP-A is a variant version of histone H3 found only at centromeres.

Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromation structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

Immunogen

peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-205

Physical form

Affinity purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

ImmunoAffinity Purified

Quality

routinely evaluated by immunoblot in acid extracted histones from HeLa cells

Specificity

CENP-A

Broad species cross-reactivity is predicted.

Storage and Stability

Stable for 1 year at 2 to 8°C from date of receipt.

Target description

17kDa